The Five X-Factors in Bacterial Protein Production and Purification

Every protein is unique and thus every protein has its own set of production and purification challenges – many of which cannot be predicted. Therefore to successfully produce and purify your favorite protein you […]

view article

Is There Overlap? Find Out with a Proximity Ligation Assay

Colocalization blues (and reds and greens) Trying to find if and where two epitopes co-localize (or, to be more precise, where they are found in close proximity) may seem easy at first: 1) Bind […]

view article

Using Flow Cytometry for Fluorescence Resonance Energy Transfer

A marriage of sorts Fluorescence resonance energy transfer, or FRET, is often done using a microscope, which means it can be difficult to analyze large numbers of cells in one sitting. One way to […]

view article

Let's Dish About Soaps: A General Overview of Detergents

What do cell lysis, clean dishes, and gallbladders all have in common? Answer: detergents! These useful chemicals can solubilize fats and other proteins in water. They are the key to applications as varied as […]

view article

Digital Pathology – why you need it and how to choose the best camera for it

Yup, we really are in the digital age…even the pathology is digital. Facebook, Instagram, Tumblr. It has never been easier to take pictures and share them. The digital revolution is upon us and nothing […]

view article

Using Word to Write your Thesis: Creating a Master Document

Wow – your thesis is really coming along. First, you created an outline to help you organize your chapters. Second, you made a Table of Contents and learned how to insert captions and how […]

view article

Your Supervisor Wants You to Apply for Funding

Whether you’re a grad student or a post-doc, the decision to apply for external funding should be a no-brainer. It is in both your boss’s and your own interests to do this and will […]

view article

Thermal Asymmetric Interlaced PCR (TAIL-PCR)

What do bunnies, coins and PCR have in common? They all have tails! (ha ha!) Thermal asymmetric interlaced PCR or TAIL-PCR is used to sequence and analyse unknown DNA fragments that are adjacent to […]

view article

Polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA)

As researchers, we are constantly on the lookout for new and improved ways to analyze, detect and quantify our favorite protein or gene. Luckily, we don’t always need to reinvent the wheel! PCR-ELISA is […]

view article

Using Word to Write your Thesis: Making a Table of Contents, Inserting Captions, and Cross-referencing

In the last post I showed you how to make an outline for your thesis in Word. You should now have a document outline with a list of headings for your sections (maybe even […]

view article
« Previous PageNext Page »

Article Categories