The Devil is in the Details: How to Setup a PCR Laboratory

There is a right way and a wrong way to set up a PCR laboratory. Because of PCR’s tremendous ability to amplify small quantities of DNA/RNA template, even the smallest of template contamination can […]

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An Easy Way to Start Using R in Your Research: Making Pretty Plots With ggplot

The thing that was most difficult for me as an R beginner was plotting graphs with error bars – there is no concise way to do this with base graphics. There are workarounds, often […]

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How Does BLAST Work?

More than a pun on the explosive growth of sequencing data, BLAST makes annotation and comparisons of similar sequences much easier. Created by a group at the U.S. National Center for Biotechnology Information in […]

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How to Check the Accuracy of Your Pipette

How much time do you spend thinking about the accuracy of your pipette? Probably not much. It’s one of those things that get brushed aside in the heat of experimentation. Pipetting accuracy though, is […]

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Do-it-Yourself PCR

Currently Open Source principles are offering interesting tools for doing molecular biology at an incredibly low cost. One interesting example is OpenPCR (www.openPCR.org) a project developed in order to ensure that the basic technology to […]

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Getting started with Immunohistochemistry

What is immunohistochemistry… Immunohistochemistry (IHC) is a favorite tool amongst clinicians to help diagnose a range of diseases by identifying abnormal cells, such as those in cancer. In a nutshell, IHC uses antibodies to detect proteins (antigens) […]

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Go Fishing for Your Favorite Protein with Immunoprecipitation!

You have your favorite protein in mind and are ready to set up some exciting experiments to show what, how, when it works, modifies, and changes in your cells. Only problem? You need to […]

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Getting the Most Out of Your Column: Optimizing Your HPLC Gradient

Let’s imagine the following scenario: You are researching a biosynthetic pathway in your favorite fungus. You know that this pathway produces a family of toxic compounds, and you want to see if you can […]

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To Pour or to Spread, This is the Question

Dear Aunt Yersinia, What are advantages and disadvantages of pour and spread plates? Antonio   It is refreshing to see such wisdom – knowing that there are at least two methods of pouring plates […]

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How You Can Use HPLC In Your Research

If you’re an HPLC guru, then you probably think that everyone should be using HPLC.  And you might have a point – HPLC is very powerful and has broad applications across many fields.  But […]

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