Do-it-Yourself PCR

Currently Open Source principles are offering interesting tools for doing molecular biology at an incredibly low cost. One interesting example is OpenPCR (www.openPCR.org) a project developed in order to ensure that the basic technology to […]

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The different phases of PCR and why they are important

PCR (Polymerase Chain Reaction) is a biochemical technique developed by Kary Mullis in 1983 that is used to create large quantities of a sequence of DNA. Since this method of mass-producing DNA was first […]

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Get Out of Western Blot Hell: An Intro to Mass Spectrometry.

  After you finish immunoprecipitating a protein or purifying a subcellular compartment, you need to identify what proteins you purified. You could attempt to identify your purified proteins the old fashioned (and slow!) way […]

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Apoptosis Gone Wrong: Cell Death’s Role in Disease

Like yin and yang, apoptosis has a duality.  While it is is a pathway used in the normal maintenance and development of tissues in healthy organisms it also had a dark side. As you […]

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How are crimes solved by PCR?

The scene of the crime The body of a woman is found behind an abandoned warehouse on the outskirts of town. Forensic experts carry out a technical examination of the scene and suggest strangulation […]

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Agarose versus Polyacrylamide: Not All Gels Are Created Equal

Like athletes running on turf versus sand, the gel you run your DNA through can highly affect your results. The two main types of gels that people use for DNA electrophoresis are agarose and […]

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How to keep your Flow Cytometry Core Facility Happy

The helpful people who work in your flow core are an amazing resource of information for your experiments and are the people with the power to get experiments done. However, you need to be […]

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Running You Through HPLC: How Does it Work?

Your advisor tells you that he wants you to use HPLC to analyze your compound. You know you’ve heard of this technique before, but you can’t remember what HPLC stands for, let alone how […]

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Gel Electro-For-Whatsit? Breaking Down How Gel Electrophoresis Works

Run to red!  It’s a mantra I learned when first using gel electrophoresis to separate DNA molecules.  This can save you a lot of frustration and humiliation in the lab (stage right: a complaining […]

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Analysing Microscopy Images? What You Should Know About Dynamic Range: Part 2

In the first part of this article (you can read it here), we looked at clipping and saturation in terms of microscope images, followed by a definition of Dynamic Range and an introduction to […]

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