An Introduction to Gating in Flow Cytometry

What is one of the first things you do when you sit down at the flow cytometer and start looking at your cells? You start drawing polygons and setting gates. To the neophyte the […]

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R You Ready? Using R for Statistical Tests

We’ve been slowly coaxing you along in our R tutorials.  We've introduced what R is, gave you a basic tutorial into how to use R and also spent some time learning how to explore […]

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Agarose versus Polyacrylamide: Not All DNA Gels Are Created Equal

Like athletes running on turf versus sand, the gel you run your DNA through can highly affect your results. The two main types of gels that people use for DNA electrophoresis are agarose and […]

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How to keep your Flow Cytometry Core Facility Happy

The helpful people who work in your flow core are an amazing resource of information for your experiments and are the people with the power to get experiments done. However, you need to be […]

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How to Switch Mentors, Part 3: Actually Switching – Is it Worth it?

Grad school is a big investment of your time, with a lot riding on a successful relationship with your mentor.  Unfortunately, you may have realized that the relationship is not working and resists improvement.  […]

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A Primer on Designing Site-Directed Mutagenesis Primers

I spent a lot of my time in the lab as an evil scientist creating mutant proteins by site-directed mutagenesis using PCR. One thing that I had to do again and again was to […]

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Gel Electro-For-Whatsit? Breaking Down How Gel Electrophoresis Works

Run to red!  It’s a mantra I learned when first using gel electrophoresis to separate DNA molecules.  This can save you a lot of frustration and humiliation in the lab (stage right: a complaining […]

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Analysing Microscopy Images? What You Should Know About Dynamic Range: Part 2

In the first part of this article (you can read it here), we looked at clipping and saturation in terms of microscope images, followed by a definition of Dynamic Range and an introduction to […]

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Get Your Proteins! Hot Proteins Here! Radioactively Labeled Proteins!

  Radioactive protein labeling is not as common as it used to be. With the advent of modern protein labeling techniques, such as fluorescence, radioactive labeling has largely fallen out of favor. However, radioactive […]

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Chasing the Pot of Gold at the End of the Rainbow: Choosing the Right Fluorochromes for Your Flow Cytometry

With the current proliferation of new dyes and instruments that can detect many colors simultaneously, it seems like an entire rainbow is at your disposal for your flow cytometry experiments. And we know that […]

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