Immunohistochemistry- Direct vs. Indirect Methods, and a Golden Rule

In my previous article I covered different immunohistochemical staining techniques at a superficial level. In the following articles I will start to explain these technologies in a bit more detail and in which situations […]

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Evolution Flow: The Historical Background of Flow Cytometry

For those of you who print out the results that you have acquired from the Flow Cytometer to stick in your lab book, did you know that inkjet printers and cytometers have a shared […]

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Do Your Homework to Find Good Reference Genes

Comparing and measuring gene expression is certainly an integral part of research—gene expression patterns continue to show us how different cell networks are regulated, and point to new biological pathways and possible treatments for […]

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How Can A Single Mutation Affect Splicing Regulation?

Alternative splicing is a highly orchestrated process that uses a multitude of regulatory mechanisms. Splicing specificity involves a precise interaction between cis- and trans-acting regulatory elements, and factors that disrupt these interactions can result […]

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How To Identify Conserved Elements In Genes

Conserved elements are stretches of DNA sequence that are under purifying selection. That means mutations leading to a change of function in this part of the DNA are detrimental to the organism and will […]

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7 Tips for Preparing Chromatin for ChIP from Tissues (Rather than Cells)

A commonly used technique in epigenetics is Chromatin Immunoprecipitation, or ChIP for short. This technique can show you whether a certain protein (e.g. transcription factor or histone modification) binds to DNA, when in its […]

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Outgrown the Roost: A Quick Protocol for Passaging Adherent Cells

Your cells have dutifully “sat down”, stuck to the bottom of the dish and replicated, replicated, replicated. You see a swarm of cells that have reached confluency and filled the entire plate. It is […]

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Tips For Better ELISA Results

The ELISA assay is a rapid method used to detect the amount of a protein of interest in experimental samples. While this process has become increasing automated and less labour intensive, it is still […]

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An Introduction To Stereology Part 1

According to the International Society for Stereology, the area of scientific study encompassed by this term is that which analyzes solids. If that all sounds a bit too much like materials science, then for […]

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How a Flow Cytometer Works: A Look Inside the Magic Box

A flow cytometer is a device used to illuminate objects and capture and quantitate light emitting from these objects.  The “objects” are normally single cells dispersed in a medium, but could very well be polystyrene beads, […]

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