The Beginners Guide to DNA Sequence Alignment

Fortunately, those of us who have learned how to sequence know that aligning sequences is a lot easier and less time consuming than creating them. Whether you’re employing sequencing gels, Sanger-based methods, or the […]

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Five Things You Might Not Know about LB

LB medium is a staple in virtually every lab. It’s commonly used to propagate E. coli, and as such will be used frequently by any lab that does cloning. Chances are, LB broth or […]

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A Short History of Sequencing Part 3: personal NGS, disposable sequencers and what the future holds.

Personalizing NGS Due to the relative ease of whole human genome sequencing, clinical researchers became interested in how they might use this technology to help their patients. There were, and still are, obstacles to […]

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DEPC: The Wicked Witch of RNA?

If you have ever worked with RNA, you know about DEPC (diethylpyrocarbonate). You add it to water at a concentration of 0.1%, shake or stir, incubate at 37°C for two hours or at room […]

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Why Pick PAS for Histology?

Periodic acid-Schiff (PAS) is another commonly used special stain in the histology lab. What Does PAS Stain? (1) Polysaccharides: The technique is commonly used to identify polysaccharides- these macromolecules are composed of monosaccharide units […]

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A Quick Guide to pH, pKa and pI (Members Only)

They’re easy numbers to take for granted, so it’s a good exercise once in a while to remind ourselves what pH, pKa and pI stand for: pH—the measure of acidity. It’s the negative logarithm […]

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Science: Why Do We Do it?

Five months ago, I pranced into the office of a renowned research institution wide-eyed and full of hope. I was about to begin my MSc project, and nothing could bring me down. I no […]

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A Short History of Sequencing Part 2: the first of the next.

The Human Genome Project was successful, but hard work. The major improvements to the technology were the increases in parallelization and automation. In 2003, just as the HGP completion papers were published in Nature […]

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Four Ways To Keep Up With Your Field If You Are Out Of A Job

Keeping up with the latest developments in your field of research is important whether you are taking a sabbatical to travel the world or your funding has finally dried up. Whatever your situation, it […]

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Excited?! Emitting light?! An introduction to fluorescent microscopy

Fluorescent-based microscopy techniques are some of the most common ways to visualize biological structures. Almost any protein- or nucleic acid-based molecule can be tagged with a fluorescent marker or dye and subsequently detected by […]

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