How To Identify Conserved Elements In Genes

Conserved elements are stretches of DNA sequence that are under purifying selection. That means mutations leading to a change of function in this part of the DNA are detrimental to the organism and will […]

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7 Tips for Preparing Chromatin for ChIP from Tissues (Rather than Cells)

A commonly used technique in epigenetics is Chromatin Immunoprecipitation, or ChIP for short. This technique can show you whether a certain protein (e.g. transcription factor or histone modification) binds to DNA, when in its […]

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Outgrown the Roost: A Quick Protocol for Passaging Adherent Cells

Your cells have dutifully “sat down”, stuck to the bottom of the dish and replicated, replicated, replicated. You see a swarm of cells that have reached confluency and filled the entire plate. It is […]

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Tips For Better ELISA Results

The ELISA assay is a rapid method used to detect the amount of a protein of interest in experimental samples. While this process has become increasing automated and less labour intensive, it is still […]

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An Introduction To Stereology Part 1

According to the International Society for Stereology, the area of scientific study encompassed by this term is that which analyzes solids. If that all sounds a bit too much like materials science, then for […]

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How a Flow Cytometer Works: A Look Inside the Magic Box

A flow cytometer is a device used to illuminate objects and capture and quantitate light emitting from these objects.  The “objects” are normally single cells dispersed in a medium, but could very well be polystyrene beads, […]

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Second Chance Saloon: How to Western Blot a Coomassie-stained gel

In her article How to Get Perfect Protein Transfer in Western Blotting, Emily Crow recommends Coomassie staining your gel after transfer to the membrane to check the quality of the transfer. A good transfer […]

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Science writing tips by Trivalent Editing

January 2013 marks the start of a series of articles by Trivalent Editing, full of tips and advice to help you improve your academic writing skills. We are a trio of experienced biomedical scientists who spent […]

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10 Ways to Improve Your Bisulfite Sequencing Results

The importance of epigenetics in biology is increasingly acknowledged (if you’re not convinced yet, read my crash course). One commonly studied epigenetic mark is CpG methylation: cytosines that are directly followed by a guanine […]

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Some Sanger Sequencing Tips and Tricks

Sanger sequencing is still a workhorse of most molecular biology labs. Even with the advent of next-generation sequencing we still need to sequence our clones and PCR products. In this article I have listed […]

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