Three Tips (and Two Tricks) for Using BLAST

The Basic Local Alignment Search Tool (BLAST) algorithm is at the heart of a free suite of online resources available through the National Center for Biotechnology Information (NCBI).  While most researchers are aware of […]

view article

Immunohistochemistry- an Introduction, Techniques and an Evolution Towards Robots!

The last two decades have seen a dramatic increase in the number of publications using immunohistochemistry (IHC) as a research tool to identify the spatial location of proteins of interest within cells, tissue sections […]

view article

Smart Questions To End An Interview On A Positive Note

I have prepped hundreds of candidates for interviews and, by extension, I have been a part of hundreds of interviews. At some point during each of these interviews, the Hiring Manager asks the candidate […]

view article

Remote Cytometry: Help from beyond!

The idea of accessing one computer from another is long established. Unfortunately we often have visions of hackers sneaking in and stealing our data when we have most to lose. However, this type of technology can aid us in a […]

view article

Tips for Choosing and Using a New Primary Antibody

Finding a good commercial primary antibody can often feel like playing Russian roulette.  Nothing is more disappointing than buying a $300 antibody that doesn’t work for your use.  There are some steps you can […]

view article

How to Calculate a DNA Primer Concentration

Calculations can be the bane of laboratory work.  Therefore, I have always looked for easy methods for getting the math done. Here are several different ways to calculate primer concentration depending on the starting […]

view article

How to Detect Alternative Splicing Variants

Alternative splicing events often occur in a spatiotemporal manner, and some are regulated by alternative splicing regulators, with striking variation across tissue types and developmental stages. Alternative splicing events are often differentially regulated across […]

view article

How To Craft The Perfect Title For Your Manuscript

Of all the words you write to prepare a manuscript, too often the most important ten or so are left as an afterthought. You’ve slaved for weeks to finish your manuscript. Through draft and […]

view article

Pyromania: An Intro to Pyrosequencing

Anyone who is involved in DNA sequencing in one form or fashion knows there are multiple ways to skin a cat: Sanger-based, next generation (NGS) and of course the new ion torrent sequencing technology. […]

view article

Antibiotics and antimycotics in cell culture: how do they work and do I really need them?

Anyone who has spent any amount of time in a cell or tissue culture lab will have experienced contamination at some point. You might not admit it, or want to admit it, but you […]

view article
more articles

Share your tips, advice & wisdom on Bitesize Bio

We gather the best tips, advice and wisdom from you guys at the bench and publish them to help each other improve in the lab.

What could you add to this collection? Click here to explore how you could contribute to Bitesize Bio.