Free PCR for 5 years (or how to make your own Taq and Pfu)

Labs across the world spend a great deal of money on commercially manufactured Taq polymerase for PCR every year. But you don’t have to: Many labs choose to save money by making their own […]

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Sifting Detritus—Extracting DNA and RNA Samples from Soil and Feces

The value of PCR to forensics has been known for a long time; but now, getting purified DNA and RNA samples from soil and fecal samples is becoming more important and commonplace as tests […]

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Congo Red- A Special Stain For Alzheimer's Disease

What is Congo red? Did you know that Congo red (CR) originally started out as a textile dye? It belongs to the azo class of dyes- synthetic compounds that are among the most popular […]

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Scientists: Can any of us REALLY multi-task?

Unlike Nick Oswald I think I can multitask in the lab. If I organize my day efficiently and perform lots of experiments and other tasks in parallel, I get more done. But there is […]

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Which Way Forward In Ultra-High Throughput Genomic Sequencing? Reference Materials And Performance Measurements

Accuracy and reproducibility In all fields of science, accuracy and reproducibility are of paramount importance and it’s never been more true than in the case of genomic sequencing where minor errors could lead to […]

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Meet your microbes: take part in the American Gut Project

“No man is an island” said  John Donne. But if he were, every healthy man or woman would be a lavish, tropical, densely populated island. Recently, I talked to a Knight wants you to […]

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Isolation of total RNA, including microRNA, from cells and tissues

Whereas DNA can survive for millennia, RNA is short-lived, which is a bummer if you are trying to isolate it. The reason – as we discussed recently – is that RNA is prone to […]

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STORM, PALM And fPALM- The Alphabet Soup Of Super-Resolution Light Microscopy

Why do I see a cloud? When you are looking at protein localization within a cell, have you ever wondered why you see a cloud of fluorescence rather that several individual fluorescent points? Well, […]

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Postdoc Interview Preparation: Sample Questions and Answers

The prospect of going into an interview for a post-doctoral position can be quite unnerving, however in my own experience the same sorts of questions come up time and time again. It is highly […]

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Ignore The Ticking Bomb At Your Peril: RNA-Seq Normalization- A Lurking Problem And It’s Solution.

You have spent days, if not weeks, at the bench setting up the treatment and control samples for that crucial experiment. You submitted your cDNA library for sequencing and after a few weeks of […]

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