Tips for Choosing and Using a New Primary Antibody

Finding a good commercial primary antibody can often feel like playing Russian roulette.  Nothing is more disappointing than buying a $300 antibody that doesn’t work for your use.  There are some steps you can […]

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How to Calculate a DNA Primer Concentration

Calculations can be the bane of laboratory work.  Therefore, I have always looked for easy methods for getting the math done. Here are several different ways to calculate primer concentration depending on the starting […]

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How to Detect Alternative Splicing Variants

Alternative splicing events often occur in a spatiotemporal manner, and some are regulated by alternative splicing regulators, with striking variation across tissue types and developmental stages. Alternative splicing events are often differentially regulated across […]

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How To Craft The Perfect Title For Your Manuscript

Of all the words you write to prepare a manuscript, too often the most important ten or so are left as an afterthought. You’ve slaved for weeks to finish your manuscript. Through draft and […]

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Pyromania: An Intro to Pyrosequencing

Anyone who is involved in DNA sequencing in one form or fashion knows there are multiple ways to skin a cat: Sanger-based, next generation (NGS) and of course the new ion torrent sequencing technology. […]

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Antibiotics and antimycotics in cell culture: how do they work and do I really need them?

Anyone who has spent any amount of time in a cell or tissue culture lab will have experienced contamination at some point. You might not admit it, or want to admit it, but you […]

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5 More iOS Apps for Scientists

Recently we’ve seen some great apps (here and here) that can be added to a scientist’s iPhone/iPad toolbox. In this next installment of iOS Apps for Scientists, let’s take a look at 5 free […]

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Cell Cycle Analysis by Flow Cytometry: Flowing your Way through Life’s Cycle

Over the past few decades the mammalian cell cycle has been well documented. Although there are lots of checkpoints as cells move through the cycle, we can very simply divide the cell cycle into […]

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What Is Open Access Anyway?

It is a truth universally acknowledged that free cheese exists only in a mousetrap, and even there it’s free only to the end user. So it’s no wonder that the traditional system of most […]

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Did Your Parents Teach You Good Manners?

Please. Thank you. Don't talk with food in your mouth. Parents all over the world work every day to instill the right types of manners in their children. We all know we should say […]

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