When Dr Harry met Dr Sally

Logically and statistically, a lab romance (= a romantic relationship between members of one lab) should not work. Most relationships fail sooner, rather than later and working in the same lab will be extremely […]

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Common Sins When Weighing Out Chemicals

You can really tell when Honours Project students start working in the lab on their projects: the pH meter probe is suddenly floating in water and the weighing area is a mess, because nobody […]

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Immunohistochemistry- PAP, APPAP and Sandwiches!

You’ll give me an (enzymatic) complex! Following on from Part 1 of this article, let’s start by having a look at the two most popular enzymatic ‘sandwich’ methods; The Peroxidase anti Peroxidase method (PAP).  The […]

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Why You Should Be Tweeting About Science

Scientists publish with the expectation that others will take their work on board to discuss it, validate it and build upon it in future publications. Unfortunately, a common mindset is that once a paper […]

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Why do relatively few women rise to the top in academia?

Fact: The vast majority of professors are male. By the time you reach the top of the ladder, only roughly 20% of professors are female In most European countries and in quite a few […]

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Do Bad Genes Beget Disease? Hey, Not So Fast!

The purpose of genetic testing is to find altered genes that could cause disease. Consequently, people could be treated, or prospective parents can make decisions about having children. However, scientists are finding that having […]

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Outgrown the Roost: Passaging Suspension Cells

Previously, you have learned about passaging adherent cells and read a quick protocol to make it happen. In this article, I will talk about passaging suspension cells. Some cells naturally live in suspension in […]

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A Step-by-Step Guide to Designing qPCR Primers

Primer design is a very important step while setting up your qPCR assay. If your primers anneal poorly or to more than one sequence, this can significantly impact the quality and reliability of your […]

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Top Tricks for Isolation of miRNAs from Plasma and Serum

MicroRNAs (miRNA) are short, non-coding RNAs involved in post-transcriptional silencing of gene expression. miRNAs can be associated with exosomes and can function as cancer-specific biomarkers. This, coupled with the fact that they are stable […]

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An Introduction to Stereology Part 2

A Biased Study It is easier to create a biased study than one without bias. However, acting in a cautious manner, outlining steps to provide guidance, and avoiding assumptions are some ways to prevent […]

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