MIQE Guidelines for Digital PCR

MIQE what's that? When writing dPCR materials and methods for a paper have you ever pondered what information you should include? This is where the MIQE guidelines will really help. Guidelines for minimum information […]

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All for one and one for all – Fluorescence Minus One Controls

Ever done a multicolour flow cytometry experiment, run all your controls, done your compensation and then started to analyse your data and realised that you can’t work out where to put your gates? To […]

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Facing Your Laboratory Freezer: Dos and Don’ts For Defrosting Day

Your stomach clenches. Sweat snakes down your torso. The world seems to slow down. You begin the long, terrifying walk down the corridor. Your mind calls out to you to, “Run! Run now!” but […]

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Don't Be Arrested for B.I.T.E. (Bunsen Ill-Treatment and Endangerment)!

B.I.T.E., or Bunsen Ill-Treatment and Endangerment, happens every day. In the time since you started reading this article, somewhere out there, a Bunsen burner has been mishandled. This is a dark subject and while […]

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Microscope Slides 101

Would you eat your spaghetti dinner without a plate? No, of course not! It would make a big mess and be ugly to look at. Instead you NEED something to put that spaghetti on, […]

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SuperSize It! Scaling Up Your Experiments

Life in the lab is easy-peasy when you are only prepping a handful of tubes. But what if you need to scale up to 10’s or even 100’s of samples? Scaling up your experiments […]

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Take Control of Your World – Five Controls for Flow Cytometry

No, this is not a call for Geeks to take over the world – just a tiny part of it – the part that ensures success in all experiments or at least a good […]

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Help: My Gal Promoter Doesn’t Work

Dear Aunt Yersinia, I work with a yeast vector where my gene is under a Gal promoter. My boss told me to grow yeast in medium with sucrose, and then add galactose to induce […]

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Dpn I The Strange

If you ever used a site-directed mutagenesis kit or ligation-independent cloning, then you also used restriction enzyme Dpn I. But what does it do and more interestingly, why? Restriction enzymes and methylases: the yin […]

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The A-Z of PCR variants

The wide range of applications of PCR has led to an ever-growing list of variants of the technique. While some are optimizations to suit specific requirements and are very similar to basic PCR, others […]

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