Using Enzymes at the Bench — Keep it in the cooler? On ice? or at RT?

Have you ever needed an enzyme but not had an ice bucket?  Have you been tempted to just grab the enzyme out of the freezer, take what you need and quickly return the tube […]

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Prussian Blue- A Histology Stain For Iron

What is Prussian Blue? Prussian blue (PB) was actually the first synthetic color to be discovered during the Industrial Revolution. It was developed accidentally in 1704 by a chemist who was trying to produce […]

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Crush Like an Elephant, Soak Like the Rain: Old-School DNA Gel Extraction

In my previous article on DNA gel extraction, I explained how most commercially available DNA gel extraction kits work. However, there was a time before our society was blessed with these convenient marvels of […]

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How to get Organised With Reference Managers for Science- Papers

My previous posts on reference mangers have discussed Mendeley and ReadCube. Today I will be discussing Papers, the reference manager I first encountered and the one favoured by many academics, including my PhD supervisor. […]

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An Introduction to Spectral Overlap and Compensation Protocols in Flow Cytometry

It strikes fear into the hearts of new cytometrists. Compensation. More fights have started over the proper way to compensate at meetings. This article will strive to shed some light on the principles of […]

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Why Is It Important To Run Your NGS Gels Consistently?

This article discusses some of the important things to consider if you are using agarose gel electrophoresis for size-selection of your NGS libraries. Gel electrophoresis is a simple and very commonly used technique in most […]

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What’s in a Number: Getting the Right Passage in Cell Culture

Getting the Right Passage Number Using an American Type Culture Collection (ATCC) reference strain for every experiment would be great, but not all that practical. So, most labs subculture their cells into a new […]

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How DNA Gel Extraction Works

Isolating pure DNA is key to many downstream applications for molecular biologists.  Isolating large quantities of pure DNA used to be a laborious task.  But thanks to commercially available kits, older methods have been […]

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The A-Z List of Things that go “Missing” in the Lab

Here is a fun list of things that you are most likely to lose to light-fingered colleagues or nocturnal ghosts of academia. The emphasis here is on fun, so as disclaimers often go, if […]

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A World Where Mathematics And Microscopy Meet: Geometric Probability In Stereology Part Two

Following on from Part 1, we’ll now take a look at the actual use of geometric probability in stereology, as well as the advantages and disadvantages of this technique. As you’ll know from the […]

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