Let's Talk About Stats: Comparing Multiple Datasets

Last week I focused on the left-hand side of this diagram and talked about statistical tests for comparing only two datasets.  Unfortunately, many experiments are more complicated and have three or more datasets.  Different […]

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Fluorescence 101: A Beginners Guide to Excitation/Emission, Stokes Shift, Jablonski and More!

You may already use fluorescence as a tool in your microscopy and imaging work, but, do you know exactly what it is? Why are certain proteins and probes fluorescent? What causes this light emitting […]

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Ten Tips for Turning Beastly Western Blots Beautiful

Western blots can be ugly. I mean down-right, horrifically, wall-of-shame ugly.  Not only can they be embarrassing to show to your colleagues, but the ugliness can obscure your results, making it impossible to interpret […]

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Ta-Da! The Magic of Taq and TA Cloning

Sometimes a clever little trick for cloning comes along that makes you just give an appreciatory “ahhh.” For me, it was TA cloning.  TA cloning is not a new technique (I am showing my […]

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What is Digital PCR?

Digital PCR (dPCR) is a quantitative PCR method that provides a sensitive and reproducible way of measuring the amount of DNA or RNA present in a sample. This method is similar to qPCR in […]

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Let's Talk About Stats: Comparing Two Sets of Data

There are so may statistical tests out there it can be difficult to determine which is the right test to use. Below is a simple diagram to help you quickly determine which test is […]

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“Where the hell did my signal go?” AKA The Problems (and Uses) of ‘Photobleaching’ in Microscopy and Imaging

Like most things in this world, fluorophores are mortal, and eventually your once bright fluorescent image will inevitably fade to black. This fading or ‘photobleaching’ of fluorescent signal can make imaging difficult, especially if […]

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Let me introduce you to ELISA…No, not the girl…The assay.

An ELISA (Enzyme-Linked ImmunoSorbant Assay) is a popular assay that uses antibodies and color change to detect proteins, peptides, antibodies or biomolecules in complex mixtures. ELISAs are popular because they are reliable, specific, easy […]

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Buying a Secondary Antibody: Why all the Choices?

So you grab a quick 5 minutes in between lectures to sit down and tackle an item on your to-do list: order a secondary antibody for an upcoming experiment.  But when you start to […]

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I Put a Smell on You: Next Generation Sequencing Sniffs Out Olfactory Signals

Chances are you spent some of your teenage years fretting about your social status. You may have even taken steps to change your status. New haircut. New clothes. These are very human behaviors: our […]

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