10 ways to improve blunt-end ligations

Blunt-end cloning involves the ligation of DNA fragments – usually between a plasmid vector and an insert – whose terminal ends are not “sticky”. Performing these ligations is notoriously difficult, particularly with large DNA […]

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5 Digital Photomicroscopy Competitions

Have you ever considered that your data is a work of art in the making? In particular, microscopy has the potential to capture the clearest, most stunning moments in life. And when a scientist […]

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How RNA-FISH can complement qPCR, and vice versa

In studies of RNA abundance and gene expression, no one technique can answer all of the questions that need to be asked. So it is necessary to use a variety of experimental methods in […]

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8 Essential papers and reference guides for Quantitative PCR (qPCR)

It can be daunting to learn about a new topic when you look at the literature and realize that thousands of papers have been published on the topic. This is especially true for techniques […]

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Open Access Vultures

If ideas are born as a heresy and die as superstition, many enterprises are born for the bettering of humanity and serve as a source of scam at a later stage. For example, a […]

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Special Stains for Histology: An Introduction and Basic Overview

Routine tissue staining Haematoxylin and eosin (or H&E- see our H&E 101 articles here and here) is the most commonly used stain in histology labs, representing the ‘bread and butter’ stain for most pathologists […]

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Choosing the right plasmid vector: A Guide for beginners

If you’re picking up a list of available plasmid vectors for the first time, it can be mind-blowing to decide on the best one for your experiment. What cloning sites do you need? What […]

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How to Make Sure You Never Lose a Lab Sample Again

It’s 11 PM…do you know where your samples are? If you dread the thought of having to hack through ice, snow and the bone-chilling depths of every freezer to locate them, flirt with frostbite […]

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Alternatives to Paraffin: Cryo and Resin Embedding and Sectioning for Histology

In most areas of research, the chances are that you have done some kind of histology and most probably used paraffin-embedded tissue. However, the process of embedding in paraffin can be a lengthy one […]

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The lac Operon as a Microscopy Tool

The lac operon is an amazing tool in molecular biology.  It has been used for decades to turn on protein expression in an inducible manner with IPTG. The result is synthesis of vast amounts […]

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