10 Tips for Working at the Bench in Developing Countries

Working in a lab in a developing country can be a unique and exciting opportunity for any scientist. It can be very rewarding, but also challenging as you navigate foreign settings to conduct your […]

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Dressing Up Your Oligonucleotide

Oligonucleotides are those smallish bits of DNA or RNA that we rely so heavily on for many of our molecular biology experiments. In their naked form, they are single, inert strands of DNA or […]

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Mediator Probe PCR

Want a PCR method that gives you high sensitivity and is cost-effective? Then you might want to try Mediator Probe PCR.  This method gives you sensitivity and limit of detection comparable to that of […]

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Safe and sound: Gloves, goggles, gowns (and more lab safety basics)

The longer you work in research, the greater chance you have of witnessing – or experiencing first hand – a hazardous situation that can lead to an accident. These are the moments that are […]

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Understand and troubleshoot PCR with The BitesizeBio Guide to PCR

Over the years, I’ve had my fair share of ups and downs in the lab. The latter quite often centering on a failed or plainly weird PCR experiment. As I’ve gone on and become […]

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Using Word to Write Your Thesis: Miscellaneous Bits and Bobs

To round off my series of articles on thesis writing, I’d like to finish with a few final Word tips and tricks that I have picked up along the way – it’s probably not […]

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The Five X-Factors in Bacterial Protein Production and Purification

Every protein is unique and thus every protein has its own set of production and purification challenges – many of which cannot be predicted. Therefore to successfully produce and purify your favorite protein you […]

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Is There Overlap? Find Out with a Proximity Ligation Assay

Colocalization blues (and reds and greens) Trying to find if and where two epitopes co-localize (or, to be more precise, where they are found in close proximity) may seem easy at first: 1) Bind […]

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Using Flow Cytometry for Fluorescence Resonance Energy Transfer

A marriage of sorts Fluorescence resonance energy transfer, or FRET, is often done using a microscope, which means it can be difficult to analyze large numbers of cells in one sitting. One way to […]

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Let's Dish About Soaps: A General Overview of Detergents

What do cell lysis, clean dishes, and gallbladders all have in common? Answer: detergents! These useful chemicals can solubilize fats and other proteins in water. They are the key to applications as varied as […]

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