Intracellular Cytokine Staining: Letting It All Build Up Inside

Cytokines, those small proteins that modulate immune cell responses, once translated are normally secreted rapidly out of the cell. So, previously we could only check the levels of cytokines secreted in the supernatant, but we wouldn't know which […]

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Ten Non-Chemical Lab Hazards and What They Do to You!

Your lab is full of non-chemical hazards that can explode, stab, kill, and – as if that wasn’t enough – bite.  Here’s a list of those hazards to remind you why Environmental Health & […]

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Next Gen Sequencing or Microarray: How to Make the Choice

You’re about to start that big project you’ve been dreaming of for years. You’ve identified a potential miracle compound and want to figure out how it affects gene expression. But how are you going […]

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Kick Start Your Research With Crowdfunding!

Like most scientists, you rely on grant monies to fund your research. Sustaining it depends on your ability to devise promising new ideas, collect new data and show proof-of-concept before writing another proposal to […]

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Cell Proliferation Round 2 And Beyond: The Dye Dilution Method

Like the legendary fight between boxers Bowen and Burke in 1893, the cell cycle in some cells goes on and on..round 1, round 2, round 3, round 4…before the final bell is rung. Nucleotide […]

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Get your stripping stripes! Find out how to strip and re-blot your Western

Westerns can be tricky and time-consuming, so make the most of your precious membranes and their proteins. Learn how to properly strip off your antibodies and re-probe with another primary antibody. Why you should […]

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The Establishment of the Nobel Prize

Let’s play a game. I’ll say a word and you say what comes to your mind. Ready? Go! Cat… Kitchen… Doctor… Airplane… Nobel… I have no idea what you said when I said cat […]

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The Devil is in the Details: How to Setup a PCR Laboratory

There is a right way and a wrong way to set up a PCR laboratory. Because of PCR’s tremendous ability to amplify small quantities of DNA/RNA template, even the smallest of template contamination can […]

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An Easy Way to Start Using R in Your Research: Making Pretty Plots With ggplot

The thing that was most difficult for me as an R beginner was plotting graphs with error bars – there is no concise way to do this with base graphics. There are workarounds, often […]

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What Matters When Choosing Your Histology Mounting Media

After successfully collecting and processing your sample, there is only one step between you and your finished slides – mounting. Correct mounting is important for good imaging results and proper sample preservation. Read below […]

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