The Basics: How Does DNA Ligation Work?

It takes a real effort to keep your basic knowledge of molecular and cell biology fresh, in addition to everything else you have to do. Wouldn't it be great to if there was a […]

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The Real Cost of Sequencing in 2014

We are now living in the era of the $1000 genome. Unfortunately, most of us are still paying significantly more than this for a genome, or an equivalent amount of data in the form […]

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Overhang PCR

Have you ever “accidently” forgotten to add the Kozack consensus sequence to the start of a coding genes? Or forgotten to include the stop codon? Did you clone something, then realize you wanted to […]

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Top Ten Science Site for Tea-Break Reading

  We all know the feeling, you have a short incubation or time point where there won’t be enough time to start something new or get anything massively productive done, but you can’t just […]

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An Essential Toolbox for qPCR Users

Today, PCR is as common a feature to the lab as pipettes and beakers. The majority of us regularly need to amplify our DNA or RNA samples, sometimes for an ‘everyday’ PCR run just […]

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What is Electron Microscopy and Its Applications?

You likely know your way around a light or confocal microscope, but what if you need to see outside the optical range of these microscopes? Enter: Electron microscopy. Electron microscopes or EMs can increase […]

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Immunophenotyping: Identifying Who’s Who in the Cellular World

Figuring out what’s what When studying cells and cell subsets (and cell sub-subsets, and so on!!) we need ways to identify and classify every single cell. This will allow us to individually analyse each population […]

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Think You Need Cells to Make Your Protein? Think Again! Use In Vitro Translation.

Do you need purified recombinant protein? But you don’t have the time or facilities for cell culture and purification? Try using in vitro translation (IVT) instead! IVT is like your own miniature protein factory. […]

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Sleigh or Wormhole: Has Santa’s Delivery Method Evolved?

We all know the traditional Christmas Eve scene of Santa flying in his sleigh through the crisp night air, pulled by his troop of reindeers. Over the years, debate has raged in the scientific […]

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A Beginners Guide to The Point Spread Function

Suppose that you have a tiny fluorescent object, such as a 10nm-diameter fluorescent bead or even a single fluorescent molecule, and you try to observe it under a fluorescence microscope.  Provided that the object […]

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