You Too Can be a MacGyver in the Lab!

A laboratory is a place where researchers spend the majority of their lives attempting to solve the mysteries of the universe; or at least the fraction dictated in their grants. However, working away at […]

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Fun With FRAP! Fluorescence Recovery After Photobleaching for Confocal Microscopy

FRAP – one of those cool-sounding techniques that you hear people say along with FLIP, FLAP, FRET and FLIM and have always wanted to try or maybe you just want to know what it […]

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What To Do If Your Graduate Supervisor Is Not Supporting You

The relationship you have with your supervisor during the course of your PhD is a critical one. Like all other personal or professional relationships it can range from being harmonious to disastrous. Choosing a […]

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Just What Do All These Additives Do?

Every PCR battle is the same: Too little amplification of your target DNA versus too much amplification of off-target DNA. But you can win the PCR battle and amaze your co-workers by mastering the […]

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Should PCR cloning be a part of your molecular cloning toolkit?

While the classic approach to molecular cloning – using restriction enzymes to excise a DNA fragment of interest – is as useful as ever, new techniques that make cloning faster, easier and more versatile […]

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A Picture Speaks a Thousand Words – Making Diagrams Simple

Figures play a central role in science not just as a way of displaying results, although this is obviously important, but also as a way of getting across complicated theories and processes in a […]

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Two Photon Confocal Microscopy: What it is and How to Use it to Your Advantage

“A two photon microscope has higher sensitivity than a normal confocal microscope, because it uses two photos instead of one!”  Yes, I can bear witness that this phrase has actually been uttered, and it […]

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The Top 10 Western Blotting Mistakes (and Solutions!)

    As bitter experience has likely taught you, not all Western blots are pretty.  Sadly this is usually due to mistakes on the experimenter’s part. While some of these mistakes are perplexing, others […]

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Introducing the 2014 Laboratory Olympics

What better way to commemorate an Olympic year than to have your very own Lab Olympics? Lab Olympics can boost lab morale when it is low or inject some fun into your daily routine*. […]

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Don’t Get Lost in RNA-seq Translation: RNA Sequencing the NGS Way

DNA sequencing (PCR, Sanger or next-generation sequencing (NGS)) is a now familiar part of any molecular biology lab. But ‘RNA-seq’, the so-called “Cinderella of genetics”, is now becoming the belle of the ball, providing […]

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