Sequencing the microbiome: re-discovering our invisible allies

Scientific breakthroughs have profound effects on how we view and treat the world that surrounds us. Especially in the microscopic level, scientific discoveries provide the only understanding we have of Nature. One such case […]

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One Fish, Two Fish, Red Fish, Blue Fish: Are You Using the Right Scale?

There are many things that vary from one scale to the next. When picking which scale to buy or use, there are a few things to take into account: the two biggies being the […]

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MIQE Guidelines for Digital PCR

MIQE what's that? When writing dPCR materials and methods for a paper have you ever pondered what information you should include? This is where the MIQE guidelines will really help. Guidelines for minimum information […]

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Facing Your Laboratory Freezer: Dos and Don’ts For Defrosting Day

Your stomach clenches. Sweat snakes down your torso. The world seems to slow down. You begin the long, terrifying walk down the corridor. Your mind calls out to you to, “Run! Run now!” but […]

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Don't Be Arrested for B.I.T.E. (Bunsen Ill-Treatment and Endangerment)!

B.I.T.E., or Bunsen Ill-Treatment and Endangerment, happens every day. In the time since you started reading this article, somewhere out there, a Bunsen burner has been mishandled. This is a dark subject and while […]

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Microscope Slides 101

Would you eat your spaghetti dinner without a plate? No, of course not! It would make a big mess and be ugly to look at. Instead you NEED something to put that spaghetti on, […]

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Take Control of Your World – Five Controls for Flow Cytometry

No, this is not a call for Geeks to take over the world – just a tiny part of it – the part that ensures success in all experiments or at least a good […]

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Help: My Gal Promoter Doesn’t Work

Dear Aunt Yersinia, I work with a yeast vector where my gene is under a Gal promoter. My boss told me to grow yeast in medium with sucrose, and then add galactose to induce […]

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Dpn I The Strange

If you ever used a site-directed mutagenesis kit or ligation-independent cloning, then you also used restriction enzyme Dpn I. But what does it do and more interestingly, why? Restriction enzymes and methylases: the yin […]

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The A-Z of PCR variants

The wide range of applications of PCR has led to an ever-growing list of variants of the technique. While some are optimizations to suit specific requirements and are very similar to basic PCR, others […]

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