Seven Things That Really Annoy Me About Tissue Culture

The tissue culture facility can be one of the most important places in the lab.  Many researchers spend hours in the hoods isolating primary cell lines and tissue, generating samples for western blot analysis, […]

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Tissue Processing For Histology: What Exactly Happens?

A procedure which needs to take place between tissue fixation and the embedding/sectioning of paraffin blocks is tissue processing. You simply can’t take fixed tissue and embed it! We have already introduced fixation in […]

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My favorite laboratory Freebie

I love free stuff. Maybe it’s because I am from the former Soviet Union, where there wasn’t enough stuff around, let alone free. Or maybe a little happiness about getting something for nothing is […]

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Keeping Track of Your Literature

Keeping track of the scientific literature you have read is just as important as reading it in the first place.  You want to have your key papers on hand for easy access.  This will […]

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This One’s Upside Down! Inverted and Stereo Microscopes in Bioscience Laboratories

Most of the microscopes you will encounter in your laboratories will be ‘upright’. In other words, they are assembled (from top to bottom) in the order of; eyepieces, objectives (on revolving nosepiece), stage, sub-stage […]

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How to scale up a PCR reaction

Scientific creativity and repetitive tasks do not go well together.  There is nothing worse than having to repeat the same task over and over again – I know somebody who quit science because of […]

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A quick tour around probe-based multiplexing qPCR

PCR has become the tool of choice for molecular diagnostics and is now a staple platform in any laboratory setting. The versatility of this method has led to a myriad of spin-off techniques, including […]

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10 Tips For Organizing Your Lab Book

Writing your lab book is easy to put off as it never seems quite as important as splitting your cells or running that gel. I’m sure, like me, you’ve said to yourself it’s fine […]

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Counterstaining for Immunohistochemistry: Choices, Choices…

When it comes to immunohistochemistry (IHC) we spend a big chunk of time figuring out which antibody to use and which concentrations to try, but how many of us take the time to think […]

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We are the Tail that Wags the Dog: Bacteria as the Dominant Life Form on Earth

Sometimes, those of us working in the biological sciences might just wonder why we're putting all that time and effort into studying whatever it is we study. For those of us working with bacteria, […]

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