Focus on isoelectric focussing

Isoelectric focussing electrophoresis (IEF) of proteins is nowhere near as popular as its cousin – sodium dodecyl sulphate-polyacrylamide gel electrophoresis aka SDS-PAGE. While in both methods the proteins are denatured, IEF is a gel-based […]

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Laser Capture Microdissection: Get it Out!

Imagine you are studying a very interesting new protein. You know in which cell type the protein is expressed, yet that specific cell type constitutes only a small minority among a large collection of […]

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The Birth Of The Epitranscriptome: RNA Gets Methylated Too!

Most biologists are aware of chemical modification of DNA in the form of 5-methylcytosine (mC), the eponymous ‘5th base’. Moving to the 6th base The fields of epigenetics and epigenomics are increasingly having an impact […]

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Keeping up With the Periodic Table

The periodic table, that ingeniously arranged display of atomic weights, numbers and elements that’s probably posted on a door way in the back of your lab, isn’t a static document. It’s changed a fair […]

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How To Fix Suspension Cells For Microscopy And Imaging

Last week I discussed how to fix adherent cells for imaging. Fixing adherent cells was easy because you can directly grow then on your cover slips. However, suspension cells – which grow suspended in […]

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Interview Techniques: Interview like a STAR

During your time interviewing for different jobs, more likely than not you will encounter employers who conduct behavioral interviews. What is a behavioral-based interview, you may ask? Behavioral interviewing is supposed to uncover your […]

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“That Belongs To Me!”: NGS Data and Security Part 2

In the first part of this article, we revisited progress in genetic testing, from mid-20th century blood-typing through to personalized medicine. The huge increase in personal health and genome data drag along security and ethical […]

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10 Easy ways to wreck your autoclave

You know how to wreck your microscope (yes, we’ve done that ourselves), and how to repurpose them when they’re old and obsolete. We’re busy brainstorming ways to destroy our centrifuge without annihilating the laboratory, […]

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How To Fix Adherent Cells For Microscopy And Imaging

Before you can image your adherent cells you need to fix them to your microscope slides. Luckily for you- this is fairly easy to do. Thanks to the fact that adherent cells are, well, […]

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How to identify protein motifs from protein sequences

Wouldn’t it be great to put your nucleotide sequence into a program and get back a 3D-structure of your protein and a full description of its functions? In theory, because the protein 3D-structure is […]

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