Should PCR cloning be a part of your molecular cloning toolkit?

While the classic approach to molecular cloning – using restriction enzymes to excise a DNA fragment of interest – is as useful as ever, new techniques that make cloning faster, easier and more versatile […]

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A Picture Speaks a Thousand Words – Making Diagrams Simple

Figures play a central role in science not just as a way of displaying results, although this is obviously important, but also as a way of getting across complicated theories and processes in a […]

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Two Photon Confocal Microscopy: What it is and How to Use it to Your Advantage

“A two photon microscope has higher sensitivity than a normal confocal microscope, because it uses two photos instead of one!”  Yes, I can bear witness that this phrase has actually been uttered, and it […]

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The Top 10 Western Blotting Mistakes (and Solutions!)

    As bitter experience has likely taught you, not all Western blots are pretty.  Sadly this is usually due to mistakes on the experimenter's part. While some of these mistakes are perplexing, others […]

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Introducing the 2014 Laboratory Olympics

What better way to commemorate an Olympic year than to have your very own Lab Olympics? Lab Olympics can boost lab morale when it is low or inject some fun into your daily routine*. […]

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Don’t Get Lost in RNA-seq Translation: RNA Sequencing the NGS Way

DNA sequencing (PCR, Sanger or next-generation sequencing (NGS)) is a now familiar part of any molecular biology lab. But ‘RNA-seq’, the so-called “Cinderella of genetics”, is now becoming the belle of the ball, providing […]

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15 Laboratory Items You Can Buy In Any Store

A variety of lab supplies can be purchased off the shelf in your neighborhood, which can save you time and money* especially when compared to the added cost of shipping, handling, and markup of […]

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Dots, Probes and Proteins: Fluorescent Labels for Microscopy and Imaging

If you remember from one of my previous articles (if not, you can read it here!), we introduced ‘fluorophores’. These are basically substances (natural or synthetic) which have the ability to absorb light at […]

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Benchside Matchmaking—Finding the Right Buffer for Your Experiment

Buffers are often taken for granted, but they can make or break an experiment.  In previous posts, we’ve talked about the wide ranges of buffers available for biological research and the characteristics of a […]

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How to quality control check your RNA samples

You finally have your RNA in hand.  Now what? The success of downstream applications, such as microarrays and quantitative RT-PCR, relies upon having high quality, intact RNA. So it is worth your while to […]

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