Go Fishing for Your Favorite Protein with Immunoprecipitation!

You have your favorite protein in mind and are ready to set up some exciting experiments to show what, how, when it works, modifies, and changes in your cells. Only problem? You need to […]

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Getting the Most Out of Your Column: Optimizing Your HPLC Gradient

Let’s imagine the following scenario: You are researching a biosynthetic pathway in your favorite fungus. You know that this pathway produces a family of toxic compounds, and you want to see if you can […]

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To Pour or to Spread, This is the Question

Dear Aunt Yersinia, What are advantages and disadvantages of pour and spread plates? Antonio   It is refreshing to see such wisdom – knowing that there are at least two methods of pouring plates […]

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Sorting Single Cells – What Do You Need to Consider?

Flow cytometer and cell sorter manufacturers have invested considerable resources to design instruments that are the “fastest in the ‘hood” either in terms of cells analyzed per second, or in total throughput. The general […]

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How You Can Use HPLC In Your Research

If you’re an HPLC guru, then you probably think that everyone should be using HPLC.  And you might have a point – HPLC is very powerful and has broad applications across many fields.  But […]

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Shooting Trouble During Cloning

As frustration goes, cloning is often up there with trying to thread a camel through the eye of a needle. You do everything carefully: prepare your vector and fragment DNA, cut them as the […]

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The different phases of PCR and why they are important

PCR (Polymerase Chain Reaction) is a biochemical technique developed by Kary Mullis in 1983 that is used to create large quantities of a sequence of DNA. Since this method of mass-producing DNA was first […]

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Red light/Green Light In Aseptic Technique: When Is The Flame OK?

My mom is a microbiologist and so I was a lot more informed about bugs than most kids. In fact, I probably gave more than one classmate nightmares with my talk of there being […]

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Should PCR cloning be a part of your molecular cloning toolkit?

While the classic approach to molecular cloning – using restriction enzymes to excise a DNA fragment of interest – is as useful as ever, new techniques that make cloning faster, easier and more versatile […]

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10 Useful Tips For Improving Your Sorting Experiments

After a very naïve start in flow cytometry thinking that published protocols will work without fault – needless to say, that did not work out in my favor – I realize now that following […]

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