Getting in Deep: How to Deep Clean a Tissue Culture Hood

One of the most exciting aspects of being a biologist is getting opportunities to examine how and why living organisms behave the way they do. We have technology that enables us to obtain images […]

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Choosing the correct enzyme

A wide variety of enzymes are available for PCR and RT-PCR and the optimal choice depends on a range of factors specific to your experiment. Some of these factors will now be explored to […]

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How to Switch Mentors, Part 2: Planning and Preparing to Switch

Much of your success and happiness in grad school depends on an effective relationship with your mentor.  Despite your best efforts, sometimes the first relationship doesn’t work out, and you need to switch mentors […]

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Analysing Microscopy Images? What You Should Know About Dynamic Range: Part 1

Ever tried to turn the volume all the way up on a small radio or small stereo system? (Hopefully you have not tried it with earphones in!) Notice how, after some point, the sound […]

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Seeing is Believing: An Introduction to Imaging Flow Cytometry

What if there was a way to take the power and speed of a flow cytometer and couple it with the resolution of a microscope? Imaging flow cytometry does just that! Flow cytometry is […]

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Better Plasmid Midipreps Part II: What Causes Low Yields?

Recently we received a question from Bitesize Bio reader Sonia after our article How to: Get Better Plasmid Midiprep Yields. She asked: "What could be the problem when one sample gives a good yield while […]

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How to: Get Better Plasmid midiprep Yields

I get many people complaining to me about poor DNA yields from commercial plasmid plasmid prep kits. In this article I will explain the main pitfalls in plasmid isolation and how to avoid them. […]

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Why You Should Talk to Sales Reps

People often joke about hiding from sales reps, but the fact of the matter is that valuable opportunities are lost when scientists disappear into thin air. Think twice before attempting your own vanishing act; […]

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How to Switch Mentors, Part 1: Recognizing Red Flags

Grad school is a long, hard, long, time-consuming, and–wait for it–long process. A bad relationship with your primary mentor can make it worse, and may even drive you away from a science career.  Unfortunately, […]

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Keeping 'em Alive! Incubators and Chambers for Live-Cell Imaging

Live-cell imaging is a powerful technique that allows you to image dynamic cell processes, such as protein trafficking, signal transduction, endocytosis, and exocytosis, etc. However, to perform your own live-cell imaging you need the […]

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