Sorting Single Cells – What Do You Need to Consider?

Flow cytometer and cell sorter manufacturers have invested considerable resources to design instruments that are the “fastest in the ‘hood” either in terms of cells analyzed per second, or in total throughput. The general […]

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How You Can Use HPLC In Your Research

If you’re an HPLC guru, then you probably think that everyone should be using HPLC.  And you might have a point – HPLC is very powerful and has broad applications across many fields.  But […]

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Shooting Trouble During Cloning

As frustration goes, cloning is often up there with trying to thread a camel through the eye of a needle. You do everything carefully: prepare your vector and fragment DNA, cut them as the […]

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The different phases of PCR and why they are important

PCR (Polymerase Chain Reaction) is a biochemical technique developed by Kary Mullis in 1983 that is used to create large quantities of a sequence of DNA. Since this method of mass-producing DNA was first […]

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Red light/Green Light In Aseptic Technique: When Is The Flame OK?

My mom is a microbiologist and so I was a lot more informed about bugs than most kids. In fact, I probably gave more than one classmate nightmares with my talk of there being […]

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Should PCR cloning be a part of your molecular cloning toolkit?

While the classic approach to molecular cloning – using restriction enzymes to excise a DNA fragment of interest – is as useful as ever, new techniques that make cloning faster, easier and more versatile […]

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10 Useful Tips For Improving Your Sorting Experiments

After a very naïve start in flow cytometry thinking that published protocols will work without fault – needless to say, that did not work out in my favor – I realize now that following […]

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Get Out of Western Blot Hell: An Intro to Mass Spectrometry.

After you finish immunoprecipitating a protein or purifying a subcellular compartment, you need to identify what proteins you purified. You could attempt to identify your purified proteins the old fashioned (and slow!) way by […]

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The Seven Deadly Sins of HPLC

Don't be a sinner!  Avoid  these sins and get the most from your HPLC run. 1.  Failing to maintain your HPLC column  There are a few cardinal sins when it comes to column maintenance: […]

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Apoptosis Gone Wrong: Cell Death’s Role in Disease

Like yin and yang, apoptosis has a duality.  While it is is a pathway used in the normal maintenance and development of tissues in healthy organisms it also had a dark side. As you […]

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