How to scale up a PCR reaction

Scientific creativity and repetitive tasks do not go well together.  There is nothing worse than having to repeat the same task over and over again – I know somebody who quit science because of […]

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A quick tour around probe-based multiplexing qPCR

PCR has become the tool of choice for molecular diagnostics and is now a staple platform in any laboratory setting. The versatility of this method has led to a myriad of spin-off techniques, including […]

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10 Tips For Organizing Your Lab Book

Writing your lab book is easy to put off as it never seems quite as important as splitting your cells or running that gel. I’m sure, like me, you’ve said to yourself it’s fine […]

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Counterstaining for Immunohistochemistry: Choices, Choices…

When it comes to immunohistochemistry (IHC) we spend a big chunk of time figuring out which antibody to use and which concentrations to try, but how many of us take the time to think […]

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We are the Tail that Wags the Dog: Bacteria as the Dominant Life Form on Earth

Sometimes, those of us working in the biological sciences might just wonder why we’re putting all that time and effort into studying whatever it is we study. For those of us working with bacteria, […]

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How to Read a Scientific Paper

Keeping up with the scientific literature in your field of interest is incredibly important. Not only does it keep you informed as to what your competitors have been working on, it will also help […]

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Immunohistochemistry Basics: Blocking Non-Specific Staining

Immunohistochemistry staining uses antibodies to detect epitopes for targeted staining and while this assay is easy in theory, in practice it is finicky! Achieving good immunohistochemistry signal-to-noise ratio involves many factors, including a good […]

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A Brief Survey of Plasmid Mapping and DNA Annotation Software

Plasmid mapping and DNA annotation software is pretty abundant these days. A quick Google search brings up dozens of hits – but how do you know which one to use? If you are like […]

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PCR is 30! Here’s How it has Rocked the World

This year marks an important birthday for molecular biologists: the polymerase chain reaction (PCR) turns 30!  This is my eulogy to celebrate life so far for the technique that has rocked the world. PCR […]

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Is it Worth Doing a PhD After a Masters?

This is a question that probably plagues every research student at some point in his/her career. The decision to pursue a PhD after getting your Masters degree is a difficult one.  A PhD is […]

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