How Does BLAST Work?

More than a pun on the explosive growth of sequencing data, BLAST makes annotation and comparisons of similar sequences much easier. Created by a group at the U.S. National Center for Biotechnology Information in […]

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How to Check the Accuracy of Your Pipette

How much time do you spend thinking about the accuracy of your pipette? Probably not much. It’s one of those things that get brushed aside in the heat of experimentation. Pipetting accuracy though, is […]

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Type IIS Endonucleases – When Nature Lends a Hand With DNA Cloning

Good news lab workers! Always hated the tedious work of designing a cloning strategy? Or maybe always dreamed of pooling all the reactions in one tube, just to save time? Thanks to Mother Nature, […]

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Do-it-Yourself PCR

Currently Open Source principles are offering interesting tools for doing molecular biology at an incredibly low cost. One interesting example is OpenPCR (www.openPCR.org) a project developed in order to ensure that the basic technology to […]

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Getting started with Immunohistochemistry

What is immunohistochemistry… Immunohistochemistry (IHC) is a favorite tool amongst clinicians to help diagnose a range of diseases by identifying abnormal cells, such as those in cancer. In a nutshell, IHC uses antibodies to detect proteins (antigens) […]

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Spring Cleaning in the Lab – How not to Have Skeletons in your Lab Closet

Most of us hate cleaning and are often hard pressed to find time to clean our homes, never mind our laboratory space. However, an annual spring clean and maintenance of a regular cleaning rota/regime […]

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Biosafety in Flow Cytometry – To Be or Not to Be…

Biosafety is one of those things many scientists don't take seriously. I would guess, that like politics, there are 40% who believe biosafety is 'over-emphasized' and 40% who swear by biosafety. 20% are undecided. […]

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Go Fishing for Your Favorite Protein with Immunoprecipitation!

You have your favorite protein in mind and are ready to set up some exciting experiments to show what, how, when it works, modifies, and changes in your cells. Only problem? You need to […]

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Getting the Most Out of Your Column: Optimizing Your HPLC Gradient

Let’s imagine the following scenario: You are researching a biosynthetic pathway in your favorite fungus. You know that this pathway produces a family of toxic compounds, and you want to see if you can […]

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To Pour or to Spread, This is the Question

Dear Aunt Yersinia, What are advantages and disadvantages of pour and spread plates? Antonio   It is refreshing to see such wisdom – knowing that there are at least two methods of pouring plates […]

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