How to quality control check your RNA samples

You finally have your RNA in hand.  Now what? The success of downstream applications, such as microarrays and quantitative RT-PCR, relies upon having high quality, intact RNA. So it is worth your while to […]

view article

Let’s Talk About Stats: Getting the Most out of your Multiple Datasets with Post-hoc Testing

So you’ve performed a test such as an ANOVA and have found that there is statistical significance in your data (lucky you!), however you now want to know where that significance lies. When you […]

view article

Apps that bring PCR to your mobile device

Mobile devices such as iPads and smartphones are increasingly being used by laboratory researchers to aid them in their research. One example is the move towards electronic lab books. As a result, app developers […]

view article

Troubleshooting RNA Isolation

Isolating RNA is one of the more finicky protocols there is, and everyone who does it has their own personal tips and tricks to successfully isolate intact RNA from their samples with consistency. Although RNA […]

view article

Can’t See the Mouse for the Mice?! Solutions to Mouse-on-Mouse Immunohistochemistry Detection

The issue of mouse-on-mouse background is only a cause for concern for the histotechnologist working within a research environment. Those working in a diagnostic setting will probably never experience this as they will be […]

view article

Do Your Proteins do a Slow Dance Together in the Dark? Find out! Learn to do a Co-IP.

Do you wonder if your favorite protein is entwined in a delicate dance with another protein? Do you wish you could shine a spotlight on your protein to determine its dance partner? Well good […]

view article

The Pros and Cons of a Life in Academic Science

The great thing about being a scientist is, well… that you get to be a scientist!  And it can be fun and rewarding. But being a scientist can be a nasty stressful business too. […]

view article

Let's Talk About Stats: Comparing Multiple Datasets

Last week I focused on the left-hand side of this diagram and talked about statistical tests for comparing only two datasets.  Unfortunately, many experiments are more complicated and have three or more datasets.  Different […]

view article

Fluorescence 101: A Beginners Guide to Excitation/Emission, Stokes Shift, Jablonski and More!

You may already use fluorescence as a tool in your microscopy and imaging work, but, do you know exactly what it is? Why are certain proteins and probes fluorescent? What causes this light emitting […]

view article

Ten Tips for Turning Beastly Western Blots Beautiful

Western blots can be ugly. I mean down-right, horrifically, wall-of-shame ugly.  Not only can they be embarrassing to show to your colleagues, but the ugliness can obscure your results, making it impossible to interpret […]

view article
more articles

Share your tips, advice & wisdom on Bitesize Bio

We gather the best tips, advice and wisdom from you guys at the bench and publish them to help each other improve in the lab.

What could you add to this collection? Click here to explore how you could contribute to Bitesize Bio.