Pimp Your Plasmid Growth Medium

I often wonder why it is that molecular biology researchers stubbornly refuse to change 4o-year old methods that, while work, are not as good as newer, faster and cheaper methods out there. I suppose […]

view article

qPCR: RNA quality and why it matters

Gene expression analysis plays a pivotal role in a wide range of studies, including biomedical analysis and diagnostics. Of all the methods available for gene expression analysis, quantitative real-time PCR (qRT-PCR) is the most rapid, […]

view article

20 Ways to Increase your Productivity

No matter how efficient you are, it's always possible to improve your productivity and improving your productivity means that you get more of the rewards you are trying to obtain: results, publications… or dare […]

view article

Saying goodbye to 454: how to choose your next NGS platform

The Rise and Fall of the 454 Sequencer  The GS20 454 sequencer, released in 2005, was the first next-generation DNA sequencer to hit the market, and its feats quickly dazzled the scientific community. As […]

view article

PIER, HIER and Mannich: Antigen Retrieval in Immunohistochemistry

When you fix your tissue samples with paraformaldehyde (PFA) the proteins in your sample become covalently cross-linked. This is good to preserve the ‘architecture’ of your tissue sample. However, this cross-linking can become a […]

view article

Overview of Protein Turnover using 35S: how to prevent trashy data and uh-oh moments

The half-life of a protein is an important factor in many molecular biology studies. If your thesis has anything to do with proteins then your graduate advisory committee will ask about half-life, so start […]

view article

Christmas gifts for the scientist in your life

Christmas is fast approaching but if you haven't yet found the perfect gift never fear, there is still time! We’ve put together some of the most fabulous gifts for those of the science persuasion. […]

view article

All sorted? Getting the best out of your cell sorting facility

If you are bringing cells to a core to be sorted there are a few things you can do to optimise your sorting and will help the core staff. Here are a few hints: 1) Know […]

view article

5 Types of Bad Boss and How to Handle Them

Science attracts so many different and quirky personalities that you are bound to have a problem with some people. But when your boss is the problem, its a big problem for you. So what […]

view article

Microscope maintenance: changing and aligning your mercury burner

Mercury burners are one of the most common light sources used in fluorescence microscopes, producing a wide spectrum of wavelengths making them a great light source for viewing your samples. But they do have […]

view article
Next Page »

Article Categories