Western Blot, ELISA or PCR; which technique should I use?

A pretty standard experiment in a molecular biology laboratory is where a scientist stimulates some cells/tissue with a stimulus of interest i.e. cytokine. They can then analyse the cellular response to the external stimulus and […]

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When Glycogen is not Your Friend – Isolating RNA from Glycogen-Rich Tissues

Bitesize Bio has had a lot to say about RNA isolation, mainly because it is one of the most anxiety-producing requirements for molecular biology; especially when you are first starting out (although isolating proteins […]

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Verhoeff-van Gieson Stain: A Special Histology Stain for Elastic Fibers

What is Verhoeff-van Gieson’s stain? Ira Van Gieson first described the Verhoeff-van Gieson (VVG) staining protocol in 1889 as a method of evaluating collagen fibers in neural tissue. Frederick Herman Verhoeff, an American surgeon […]

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You did a Co-IP…now what?

You spent the last few weeks tweaking your Co-immunoprecipitation conditions, testing different antibody/bead combinations, and sampling a panaply of solutions and FINALLY! You have your Co-immunoprecipitation (Co-IP) elution… Now what? Well, you have a […]

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Herzenberg and the Invention of the FACS Machine

The flow cytometer that we have all grown to know and love may have only come into its own in the 1990’s, but who would have known that the first cell sorter was invented […]

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Freeze-Thaw Cycles and Why We Shouldn’t Do It

Freeze-thaw – you know it’s bad for your samples, don’t you?  While working in the lab, you are sure to have heard someone say ‘aliquot it to avoid too many freeze-thaw cycles’ but do […]

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BioBricks: Lego for Scientists!

Lego bricks are used, mostly by children, to construct vehicles, buildings, and even working robots. Surely the idea of bringing Lego to the lab is childish, but think just for a second… since the […]

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You Too Can be a MacGyver in the Lab!

A laboratory is a place where researchers spend the majority of their lives attempting to solve the mysteries of the universe; or at least the fraction dictated in their grants. However, working away at […]

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Fun With FRAP! Fluorescence Recovery After Photobleaching for Confocal Microscopy

FRAP – one of those cool-sounding techniques that you hear people say along with FLIP, FLAP, FRET and FLIM and have always wanted to try or maybe you just want to know what it […]

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What To Do If Your Graduate Supervisor Is Not Supporting You

The relationship you have with your supervisor during the course of your PhD is a critical one. Like all other personal or professional relationships it can range from being harmonious to disastrous. Choosing a […]

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