When purifying a protein, it’s important to keep your protein happy; that usually means that the protein is soluble and active. Creating a buffer that prevents unfolding and aggregation is therefore crucial to the success of your experiments. There are several factors that you should consider when designing a buffer: pH, buffering system, salt, reducing [...]
Touchdown PCR: A Primer and Some Tips
When I first heard of touchdown PCR, I thought of a landing aircraft, which, as it turns out is not a bad way to think about it. But despite it’s amenability to analogies and dreadful puns (see title), touch-down PCR (TD-PCR), a very useful technique for improving PCR amplification specificity, is trickier that it might [...]
