Stereo (Dissecting) Microscopes 101

  Think a stereo microscope might be helpful? But you don’t know how to use one? Lucky for you I wrote this primer on stereo microscopes to help you figure out when and how […]

view article

Have I Got Good Protein Expression News for You

While the art of reading hand poured sequencing gels is forgotten as the next generation sequencers spit out genome after genome, protein expression still often requires sweat, tears and a lot of luck. I […]

view article

Taken for Granted: Position and Setup of Your Microscope

Although I say ‘taken for granted’, over the years of working in and managing microscope facilities, it quickly became clear that the position of the microscope, user position and set-up were aspects which were […]

view article

The History of PCR

As with some of the greatest discoveries in science, from penicillin to microwave ovens and play-doh, PCR was discovered serendipitously. Thanks to the work of many scientists, including Watson and Crick, Kornberg, Khorana, Klenow, […]

view article

How To Calculate The Number Of Molecules In Any Piece of DNA

It’s the dreaded topic, isn’t it? Calculating the number of molecules, the concentration of a solution, or working out serial dilutions were tasks that always filled me with dread as an undergrad. And to […]

view article

What Can NMR Do For You – Part Three

Welcome to part three of “What Can NMR Do For You” This is an ongoing series in which we describe how you can use NMR experiments to characterize your protein of interest. In part […]

view article

Beware The Bane of Batch Effects

A promising study on using gene expression to develop personalized treatments for ovarian cancer. A report of surprisingly high levels of differential gene expression among different ethnic groups. The announcement of previously unsuspected levels […]

view article

Overlap Extension PCR Cloning

If efficient cloning is what you are after, you must give Overlap Extension PCR Cloning a go! This restriction enzyme and T4 DNA ligase-free technique is faster, more reliable and easier to troubleshoot than […]

view article

The Who’s Who of Super Resolution Microscopy, Part 1

The 'diffraction limit' of a microscope is the minimum distance between two fluorophores where they can be still be discriminated as two separate objects. This diffraction limit has long constrained attempts by biologists to […]

view article

Sequencing the microbiome: re-discovering our invisible allies

Scientific breakthroughs have profound effects on how we view and treat the world that surrounds us. Especially in the microscopic level, scientific discoveries provide the only understanding we have of Nature. One such case […]

view article
Next Page »

Article Categories