Understand and troubleshoot PCR with The BitesizeBio Guide to PCR

Over the years, I’ve had my fair share of ups and downs in the lab. The latter quite often centering on a failed or plainly weird PCR experiment. As I’ve gone on and become […]

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Using Word to Write your Thesis: Making a Table of Contents, Inserting Captions, and Cross-referencing

In the last post I showed you how to make an outline for your thesis in Word. You should now have a document outline with a list of headings for your sections (maybe even […]

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Insane in the Membrane! PVDF vs. Nitrocellulose – Which One Comes Out on Top?

When it comes to Western blotting, there’s no denying it: Your membrane is a key player. After all it is the physical scaffold that holds your precious samples and it needs to be up […]

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Part 2: The Who’s Who of Super Resolution Microscopy – Single Molecule Localisation techniques

In part 1 of The Who’s Who of Super Resolution Microscopy I discussed one way to overcome the diffraction limit of light: ensemble technique. In this article I discuss another way to improve resolution: […]

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Where Are My Cells: Part 2

The golden rule of flow cytometry, especially cell sorting is: ‘Put good cells in and get good cells out’. When you sort you might not get good cells out and you may not get […]

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Which Cytokine Will I Get? How to Stimulate Human Cytokine-Producing Cells

Cells are like people: depending on their current environment, past experiences and their genetic make-up they will react differently. Treat cells in different ways, and they will produce different cytokines. There are a lot […]

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Assembling the Puzzle: Cloning with Compatible Cohesive Ends

Consider a jigsaw puzzle. While most of the pieces have a different picture on their surface, all pieces fit together in an interlocking pattern. As unlikely as it may seem, restriction enzymes from different […]

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Using Word to Write your Thesis: Creating an Outline

When I started the third year of my PhD, I was advised to attend a course to learn to how to use Microsoft Word properly before I started writing. I laughed! Of course I […]

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Using Flow Cytometry for Cell Proliferation Assays: Tips for Success

We all know, generally from bitter experience, that experiments don't always work first time and that sometimes the little things that govern success are the things that get left out of that online protocol! […]

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Tips for a Happily Functioning Tissue Culture Room

You walk into your tissue culture room to find a window open, the incubator’s humidity tray empty and a pipette lying on its side in the hood on a used glove… After you have […]

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