Search results for: social media
The Why and How of Promoting Your Science Publication Online.
…paper: by email (to colleagues and peers) and of course on your personal social media accounts. Your institution may have social media accounts too, so you might ask them to…
Read MoreHow to be a Scientific Social Media King/Queen!
…of social media. Emerge as a trailblazer in communicating and promoting your hidden treasure to the world! Create an Infographic for your Social Media Page Interact, interact, interact, now and…
Read MoreIntroduction to Yeast Media: How to Feed Your Fungus
…what you will use to select plasmids. In general, it can be split into rich media and minimal media. Rich Yeast Media Rich media are the ideal choice for yeast…
Read MoreWhat’s in Your Cell Culture Medium?
…Type of Cell Culture Media So now you know what is in most cell culture media, let’s discuss some of the most commonly used media. Table 1 summarizes some of…
Read MoreHow To Passage Cells in Culture
…acidic. To remove the old media, centrifuge at 150 x g for 5 minutes, remove acidic media, gently resuspend the cell pellet in warm media, and reseed in fresh media. …
Read MoreHow to Share Your Journal Articles Legally
…social media profiles. But are you allowed to share the full text? The answer isn’t always straightforward. This article explains why sharing your journal articles can be problematic and elucidates…
Read MoreDoesn’t Play Well with Others- The Chemistry of the Autoclave
…Terrific Broth (TB), Plasmid DNA Media (PDMR), and Autoinduction Media (ZYP-5052). These media formulations optimize E. coli cell growth and performance utilizing specialized carbon sources and phosphate buffering systems, and…
Read MoreGoing Serum-Free: The How and Why of Removing Serum From Your Media
…critical aspect of cell culture, and many media recipes and commercial media preparations are the results of years of refinement. One often-used supplement in cell culture media is serum, commonly…
Read MoreScience and the Media – Dos and Don’ts
Have you ever wondered how the media can write (often cringingly inaccurately) about a recently published scientific paper? Attending Standing up for Science media workshop organised by the Sense about…
Read MoreA Beginner’s Guide to Lentiviral Transduction
…complete media to the same plate. Collect media again after 72 hours and combine with the media collected at 48 hours. Filter sterilize the combined collected media by passing it…
Read MoreBuffering Your Life with Wellness
…wellness is a feeling of connectedness to, and support from, friends and family. Social connectedness is important for surviving grad school. A strong social network can help motivate you and…
Read MoreStand up for Science: How to Detect and Fight Misinformation
…them in the news and on social media. Below are four simple steps to identifying misinformation. 1. What is the Source? The first step is to identify the source. Social…
Read MoreReproducibility 101: How to Make Media
…best foot forward with making media. The Chemicals Solutions and media are the cornerstones of your experiment. Botch that and you will not get a reproducible result or will not…
Read MoreTop Tips for Yeast Microscopy
…fluorescence microscopy: Autofluoresence from Media Rich media such a YPD is great for growing yeast, however if you are interested in flurorescent imaging this media isn’t a friend. This is…
Read MoreAlphabet Soup for Bacteria!
…animal milk or meat and are used in a number of different nutrient media for the growth of bacteria (also used in fungal growth media). R. Ribose – A sugar…
Read MoreStaying Alive: Tips for Air-Liquid Interface Cultures
…up the nutrients in their media after a few days, and so the media will need to be changed. After the cells have been established at ALI, they cannot be…
Read MoreTaking up the Challenges of In Vitro Monoclonal Antibody Production
…recombinant cell lines, the choice of culture media influences cell growth and, thus, antibody quality and yield. Laboratories often test a number of different media compositions for the cell line…
Read MoreA History of FBS: A Timeline of Cell Culture Media
…media. Before FBS 1882: Sydney Ringer became the first biologist to cultivate animal tissues in vitro. He was able to keep dissected frog hearts beating in a balanced salt solution…
Read MoreAntibiotic Disposal in the Lab: Simple Tips to Get it Right
…unused media, autoclaved media, old antibiotic stocks)? Have you ever considered if you are disposing of them appropriately? Read on to know what proper antibiotic disposal in the lab looks…
Read MoreHow to Reconcile Being an “Aspie” and a Scientist
…earphones to listen to relaxing music to escape the noise and excessive movement around you. 4. Neurodiversity and Social Convivence Because my different way of being and perceiving social rules,…
Read MoreHow to Stay on the Margin of Academia During Your Gap Year(s)
…own funding. Social Media: Twitter, Research Gate, LinkedIn, and so on Social media is a powerful tool for connecting people with similar interests, regardless of location. Want to see which…
Read MoreMastering the Art of Growing THP-1 cells
…attempt to split them. If they require media, add media to the flask. But keep some of the original media as this contains growth factors which will help the cells…
Read MoreBasic Bacterial Culturing Practices
…media is typically used. Microliter-sized cultures can easily be up-scaled to larger cultures as required. Liquid media is easy to prepare in large amounts, and growth parameters such as the…
Read MoreWant to Increase Your Lentiviral Titers? Focus on Your 293T Cells
…bottom corner and add your media there. Now, pipette your trypsin-media mix directly onto your cell monolayer. Don’t blast the cells off the plastic, they should detach easily. If you’re…
Read MoreInfecting Eukaryotic Cells With Bacteria (On Purpose!)
…cultured in antibiotics, replace the media with antibiotic-free media before infection. If you ordinarily add antimycotics or other additives to your cell culture media, try to find out if these…
Read MoreHow to Reduce Your Use of Animal Products in the Lab
…with formulating and screening serum-free media. [5] [6] The FCS-free database has a wealth of information on various FCS- and animal-free media along with formulae to make in-house serum-free media….
Read MoreFive Ways to Help You Achieve Happiness in Graduate School
…something that you love you boost your happiness levels and your productivity 4. Don’t neglect your personal and social life. Studies show that everyone feels happier when they socialize, including…
Read MoreOptimal Conditions for Live-Cell Imaging
…suitable brain imaging microscope. Growth media or saline-based media formulations such as DMEM, RPMI, and Leibovitz L-15 are widely used in live-cell imaging experiments. The growth media solutions are formulated…
Read MoreSuccessful Start: Tips for Establishing Organoids
…of organoid experiments. There are preformulated growth factor mixes that can be added into media stocks and premade bottles of media available commercially, although this is for a limited range…
Read MoreA Step-by-Step Guide to the Quantitative Beta-galactosidase Assay for Y2H Screening
…cells and wash with cold sterile water to remove any traces of glucose. Transfer the cells in induction media (appropriate drop-out media + galactose) and let them grow for at…
Read MoreWhy You Should Defend Science
…style of writing in academic journals is snooze-inducing, even within your own field! Therefore, universities that are keen for public exposure release interesting findings to the media. The media report…
Read MoreGet Great Yields by Optimizing Your Bacterial Cultures
…MgCl2 or MgSO4 can improve the growth of your cultures, especially if you use basic media such as LB broth. Some other media, such as SOB and SOC already contain…
Read MoreHow To Fix Adherent Cells For Microscopy And Imaging
…cell line Pipettes and tips Growth media Freezer Methanol or paraformaldehyde Cell incubator Ethanol Cell culture hood Sodium hydroxide Large beakers Phosphate-buffered saline (PBS) 24-well plates Poly-lysine or gelatin Coverslips…
Read MoreTop 5 Tips for Maintaining Your Sanity During Thesis Writing
…the window when you’re thesis writing is your social life. Even if you have no responsibilities other than to get your thesis written and submitted on time, your social life…
Read MoreUnder Pressure: How to Stay Sane in a Publish-Or-Perish World
…from social media, perhaps on a regular basis. But that doesn’t mean withdrawing completely from social connection. Reach out to the friends you really want to speak to. Sometimes silence…
Read MoreOverview of Protein Turnover using 35S: how to prevent trashy data and uh-oh moments
…chase media should be double the volume of the pulse media and should contain an excess of the cold (not radioactive) amino acids used in labeling (around 15 mg/L). When…
Read MoreResolution and Refractive Index: Set-up Your Confocal Wisely!
…Oil 1.51 Glass 1.52 Mowiol (Mounting Media) 1.41-1.49 Vectashield (Mounting Media) 1.46 Flourmount (Mounting Media) 1.40 Fresh Prolong Gold (Mounting Media) 1.39 1-day-old Prolong Gold 1.40 4-day-old Prolong Gold 1.44…
Read MoreBeginners Guide to Setting Up Migration and Invasion Assays
…cell growth during starvation and treatment beforehand if necessary. Starving cells in media containing 0.1% FBS synchronizes the cell cycle and depletes growth factors that could influence cell migration. GOLDEN…
Read MoreSlides 101
…#1 at 0.13 mm to 0.16 mm) and anticipate your mounting media adding 1–2 ? m of thickness. Mounting Medias Dry (no media). Dry mounting is the simplest way to…
Read MoreHow to Preserve Microorganisms: Store Your Cells Better
…media (bacteria and algae), growth medium containing the bacterial hosts (bacteriophages), or water (fungi and yeasts) before inoculating into fresh culture media. Micro-encapsulation In micro-encapsulation, cells are entrapped in a…
Read More