Today’s issue of the Biotechniques journal is well worth a read. The journal celebrates 25 years of existence with a series of retrospective articles covering developments in various fields over the same period.
If you are regularly doing ChIP-qPCR, ChIP-RNAseq or luciferase reporter assays to measure protein-DNA interactions, then this article is for you! ChIP experiments can tell you what DNA sequences your protein binds, and luciferase reporter assays predict whether your protein functionally binds a specific promoter to activate transcription – but a yeast one-hybrid (Y1H) assay […]
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