Today’s issue of the Biotechniques journal is well worth a read. The journal celebrates 25 years of existence with a series of retrospective articles covering developments in various fields over the same period.
Here’s a tip that you may find useful if you are expressing proteins in E.coli using a lac promoter-based expression system, e.g. pET, in LB medium (L-broth). Lac expression systems are typically induced in the lab using IPTG (isopropyl-beta-D-thiogalacto- pyranoside), which is a non- hydrolyzable analog of lactose, the natural inducer of the lac operon. […]
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