John’s comment on Jode’s recent article here on Bitesize Bio: “Good idea on marking the rotor for 3 tubes Jode. One of those tiny (perhaps tragic) pleasures is when you drop the 3 tubes in quickly and get in spaced perfectly first time. Because usually its drop them in and then move one tube 1 space over and then 1 other tube 1 space back.” made me laugh in recognition.
How many of us can identify with that small surge of pleasure when something tiny and insignificant goes right in the lab? Here are a few things that will make me smile any day:
getting tubes balanced in the microfuge on the first try (so right, John!)
weighing out a sample to exactly 20.00 g on the balance
having just enough paper in the printer to print out an article
entering sample 1000 in the -80°C database
grabbing the right number of tubes for all your samples without counting
ejecting all 8 tips from the multichannel pipettor at the same time
finding the microscope already set to the right objective and filter
having enough different Sharpie colors to label all your different samples
dividing a piece of tinfoil exactly to cover bottles for autoclaving, with no scraps left over
PCR screening is great for identifying potential positive clones after cloning/subcloning. However, you’ll still want to confirm that the DNA sequence is correct for any positives identified. The easiest and quickest way to do this is by restriction digestion. This guide will tell you about the theory and practice of using this technique. The Theory […]
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