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ECL can be an expensive reagent in a lab, and what with it being involved in the final stage of a western blot, it’s something you don’t want to have to worry about too much. During my PhD, I was struggling with my western blots for ages – it seemed I was doing everything right, [...]
Antibiotics are used in a wide range of techniques in molecular biology. My aim with this post is to provide an easy reference to some of the main antibiotics used in molecular biology, their mechanisms, range and working concentrations. I hope you will find it useful. If I have missed out an antibiotic that you [...]
How to tell which identical twin is the father of your child, CRISPR arrays helping bacteria fight off phages and patents written on toilet paper. It’s all happening in this week’s look around the blogs… Awww… he has your DNA Copy Number Variation Profile Hsein Hsein at Eye on DNA discusses how to tell the [...]
Cell-free protein synthesis (aka In vitro translation) refers to protein production in vitro using lysates generated that provide the cellular machinery necessary for synthesis. The lysates can be of bacterial or eukaryotic origin. It is a useful alternative to in vivo synthesis for generating protein for the study of things like: protein:protein interactions (pull-down assays, [...]