Comments on: The Basics: How Alkaline Lysis Works http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/ Daily tech tips, news and comment for molecular and cell biologists Wed, 08 Sep 2010 22:09:58 +0000 hourly 1 http://wordpress.org/?v=3.0-beta2-14565 By: sameer thukral http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-19801 sameer thukral Wed, 07 Jul 2010 17:38:13 +0000 http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-19801 Monisha out there asked a lot of days ago why we need glucose in solution I? Any answers as yet? Monisha out there asked a lot of days ago why we need glucose in solution I?
Any answers as yet?

]]> By: sameer thukral http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-19800 sameer thukral Wed, 07 Jul 2010 17:38:10 +0000 http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-19800 Monisha out there asked a lot of days ago why we need glucose in solution I? Any answers as yet? Monisha out there asked a lot of days ago why we need glucose in solution I?
Any answers as yet?

]]> By: sameer thukral http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-19798 sameer thukral Wed, 07 Jul 2010 17:38:09 +0000 http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-19798 Monisha out there asked a lot of days ago why we need glucose in solution I? Any answers as yet? Monisha out there asked a lot of days ago why we need glucose in solution I?
Any answers as yet?

]]> By: sameer thukral http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-19799 sameer thukral Wed, 07 Jul 2010 17:38:09 +0000 http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-19799 Monisha out there asked a lot of days ago why we need glucose in solution I? Any answers as yet? Monisha out there asked a lot of days ago why we need glucose in solution I?
Any answers as yet?

]]> By: How Silica Spin Column DNA and RNA Preps Work | Bitesize Bio http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-19747 How Silica Spin Column DNA and RNA Preps Work | Bitesize Bio Mon, 28 Jun 2010 11:08:27 +0000 http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-19747 [...] not added until after lysis and the salts are used for binding. An excellent in-depth article on alkaline lysis is here and also another article on the difference between genomic DNA and plasmid is available for further [...] [...] not added until after lysis and the salts are used for binding. An excellent in-depth article on alkaline lysis is here and also another article on the difference between genomic DNA and plasmid is available for further [...]

]]> By: Pranay Dogra http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-19589 Pranay Dogra Mon, 07 Jun 2010 18:27:50 +0000 http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-19589 Excellent article. The discussion is even better. I had all my doubts regarding Plasmid DNA extraction cleared today. I have to deliver a lecture a on genomic DNA extraction and these concepts will come in handy answering the questions of the students. Thanks. You are a life saver Nick :-) Excellent article. The discussion is even better. I had all my doubts regarding Plasmid DNA extraction cleared today. I have to deliver a lecture a on genomic DNA extraction and these concepts will come in handy answering the questions of the students.

Thanks. You are a life saver Nick :-)

]]> By: Watanyoo Sopipong http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-19223 Watanyoo Sopipong Wed, 12 May 2010 14:02:00 +0000 http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-19223 Thank you ;D Thank you ;D

]]> By: Jode http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-18530 Jode Fri, 26 Mar 2010 06:01:27 +0000 http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-18530 Yoo, While phenol and chloroform have low dielectric constants, they are almost completely immiscible in water. Therefore, the dielectric constant of the aqueous phase (where the DNA is) is minimally effected during an extraction. Since small alcohols are miscible in water, they can drop the dielectric constant and precipitate DNA. Yoo,

While phenol and chloroform have low dielectric constants, they are almost completely immiscible in water. Therefore, the dielectric constant of the aqueous phase (where the DNA is) is minimally effected during an extraction. Since small alcohols are miscible in water, they can drop the dielectric constant and precipitate DNA.

]]> By: Jode http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-18529 Jode Fri, 26 Mar 2010 05:52:35 +0000 http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-18529 Sam M, Strictly speaking, E. coli aren't soluble, so they scatter light. Once you add the lysis solution, the cells pop and all the components of E. coli are solublized (surrounded by water molecules), either by the water directly, or with the detergent's help, and the solution clears. Sam M,

Strictly speaking, E. coli aren’t soluble, so they scatter light. Once you add the lysis solution, the cells pop and all the components of E. coli are solublized (surrounded by water molecules), either by the water directly, or with the detergent’s help, and the solution clears.

]]> By: Jode http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-18528 Jode Fri, 26 Mar 2010 05:37:09 +0000 http://bitesizebio.com/2007/11/07/the-basics-how-alkaline-lysis-works/#comment-18528 Ranga, I'm a biochemist, which is kinda-like a chemist. Outside of that most of what I've learned came about after having something go horribly wrong, and then trying to figure out why. I've also been pretty lucky and frequently have had people around me that could help fill in the gaps in my knowledge. Hopefully, this web site can help others who don't have that go-to person down the hall. Ranga,

I’m a biochemist, which is kinda-like a chemist. Outside of that most of what I’ve learned came about after having something go horribly wrong, and then trying to figure out why. I’ve also been pretty lucky and frequently have had people around me that could help fill in the gaps in my knowledge. Hopefully, this web site can help others who don’t have that go-to person down the hall.

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