If you do a lot of heterologous protein expression, take a look at Eurogentec’s CherryExpress Kit. Based on the T7 expression system, the CherryExpress vector has a sequence encoding a small red polypeptide (the heme binding part of cytochrome) fused to the promoter. When your favorite gene is cloned in, the resulting fusion protein is bright red, allowing easy identification of clones expressing your gene. Even better, protein expression level can be quantified at 480nm and the highly soluble peptide could even improve the soluble expression of your protein. Tasty.
Effective sterilisation techniques are essential for working with isolated cell lines for obvious reasons you don’t want bugs from the environment growing in your nice culture medium, and equally, cultures must be sterilised before disposal. So what are the most common methods of sterilisation, and how do they work? Unsure? Read on… WET HEAT (Autoclaving) […]
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